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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: Circulating small extracellular vesicle-encapsulated SEMA5A-IT1 attenuates myocardial ischemia–reperfusion injury after cardiac surgery with cardiopulmonary bypass

Fig. 2

SEMA5A-IT1 shuttled in post-sEVs protects against myocardial H/R injury. A The uptake of sEVs by human AC16 cells. B RT-qPCR was performed to examine the expression of SEMA5A-IT1 in AC16 cells co-cultured with pre-sEVs or post-sEVs. C The expression of SEMA5A-IT1 in AC16 cells transfected with si-SEMA5A-IT1. D The expression of SEMA5A-IT1 in AC16 cells infected with SEMA5A-IT1-overexpressing lentiviral vectors. E The expression of SEMA5A-IT1 in AC16 cells infected with SEMA5A-IT1-overexpressing plasmids and exposure to H/R damage. F Cell viability was detected in different groups by CCK8 assay. G Cell apoptosis was detected in different groups by flow cytometry of Annexin V/IP staining. H Western blot was used to detect Bcl-2 and Bax protein expression. ***p < 0.001 compared with the control group. I ROS, MDA, GSH, and iron accumulation were detected in each group. #p < 0.05, ##p < 0.01, ###p < 0.001 compared with the SEMA5A-IT1_OE group

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