Fig. 3

SEMA5A-IT1 sponges miR-143-3p and negatively regulates the expression of miR-143-3p. A AC16 cells were incubated with labeled sEVs for 4 h and then stained with DAPI. The uptake of sEVs was observed under a fluorescence microscope. Scale bars, 50 μm. B The putative miR-143-3p bound sequence of the wild-type (wt) and mutant (mut) sequence of SEMA5A-IT1 from the miRDB database. C Luciferase reporter assay was conducted to verify the binding relationship between SEMA5A-IT1 and miR-143-3p. D The Ago2-RIP assay was conducted to reveal that both endogenous SEMA5A-IT1 and miR-143-3p could be pulled down by the AGO2 antibody. E RT-qPCR was used to detect the expression of miR-143-3p in AC16 cells after being infected with SEMA5A-IT1-overexpressing plasmids and exposure to H/R damage. SEMA5A-IT1-wt, SEMA5A-IT1 wild type; SEMA5A-IT1-mut, mutant SEMA5A-IT1. *p < 0.05, **p < 0.01, ***p < 0.001, and ns indicates no significant difference