Fig. 5

SEMA5A-IT1 protects cardiomyocytes from H/R damage by upregulating BCL2 and SLC7A11. A RT-qPCR was performed to measure the levels of BCL2 and SLC7A11 in AC16 cells transfected with siRNAs targeting BCL2 or SLC7A11. Next, after AC16 cells were co-transfected with si-BCL2, si-SLC7A11, or miR-143-3p mimic and SEMA5A-IT1-overexpressing plasmids, they were exposed to H/R condition. B–G RT-qPCR to measure the levels of BCL2 and SLC7A11 mRNA (B), western blot to assess the levels of BCL2 and SLC7A11 protein (C), CCK8 assay for cell viability (D), flow cytometry for cell apoptosis (E, F), and detection of ROS, MDA, GSH, and iron accumulation (G). **p < 0.01, ***p < 0.001 compared with the H/R group. #p < 0.05, ##p < 0.01, ###p < 0.001 compared with the SEMA5A-IT1_OE group