Fig. 5

Expression of Lr-pgrn-s1 after lamprey skin and spinal cord injury. A Expression of Lr-pgrn-s1 in lamprey skin lesions 1d and 2d. B Expression of Lr-pgrn-s1 in lamprey spinal cord injury at 6 h, 1d and 3d by qRT-PCR. C Detection of tissue localization of Lr-PGRN-S1 in skin injury 1d by immunohistochemistry. Take pictures at 40× magnification. D Immunohistochemistry of Lr-pgrn-s1 was knocked down 6 h after lamprey spinal cord injury. Results under 20× and 40× microscopy in Sham, NC (normal control) and Lr-PGRN-S1 groups. E The qRT-PCR analysis of genes in MAPK, Notch, Wnt and TGF-β signaling pathways. The statistical differences between experimental groups were detected by the Student’s t test. All data were presented as the means ± SDs based on three independent samples with three replicates per sample. ns: non-significant, *P < 0.05, **P < 0.001, ***P < 0.0001