Fig. 6

High-resolution 3D tomography showing post-MCAO brain vasculature under different Xbp1 interventions. A 3D surface volume images rendering the general appearance and leptomeningeal vasculature of rat brain under NC, ov-Xbp1, and sh-Xbp1 conditions. Scale bar, 1000 μm. B 3D vasculature images rendering the path through vessel segment and vascular interconnections under NC, ov-Xbp1, and sh-Xbp1 conditions in the whole-brain context. The red regions indicate the highly connected microvessel network, which were usually seen at the lesion. Scale bar, 1000 μm. C 3D vasculature images showing the diameter of the vessels under NC, ov-Xbp1 and sh-Xbp1 conditions in the whole-brain context. The pseudocolor from blue to red indicates the vessels of 10–100 μm in diameter. Scale bar, 1000 μm. D 3D images showing the vessel surface area of the regions of interest (ROI) under NC, ov-Xbp1, and sh-Xbp1 conditions. The outline of the brain and important structures is also displayed. The lesion is labeled in light-blue color. Scale bar, 1000 μm. E Comparison of the whole-brain vascular nodes in the NC, ov-Xbp1, and sh-Xbp1 groups. Results are presented as mean ± SD (n = 3 in each group). One-way ANOVA followed by Tukey’s multiple comparisons test. ****P < 0.0001 compared with the control; ^####P < 0.0001 compared with the ov-Xbp1 group. F Comparison of the segments of blood vessels in the NC, ov-Xbp1, and sh-Xbp1 groups. Results are presented as mean ± SD (n = 3 in each group). One-way ANOVA followed by Tukey’s multiple comparisons test. ****P < 0.0001 compared with the control; ^####P < 0.0001 compared with the ov-Xbp1 group