Fig. 1

Identification of LSSL in lamprey serum. A Transmission electron microscopy (TEM) of membrane proteins from Escherichia coli, rabbit red blood cells (RRBCs), and HeLa cells treated with lamprey serum. Scale bar, 50 nm. B SDS–PAGE of membrane proteins extracted from E. coli treated with lamprey serum. The membrane proteins extracted from PBS-treated E. coli were used as the control. Protein bands (red box) were identified using mass spectrometry. C Detection of native LSSL protein purified from lamprey serum using SDS–PAGE and Native–PAGE. The molecular weight of the LSSL monomer is about 35 kDa. D SDS–PAGE of recombinant LSSL protein, the molecular weight of which is approximately 38 kDa