Fig. 4
LSSL has strong bacterial agglutination effect. A ELISA showing the interaction between LSSL and microbial components and glycans. Plates were coated with 20 μg components, washed, and incubated overnight with LSSL at 4 °C, followed by detection using an anti-LSSL antibody. One representative experiment of three is shown (n = 3 technical replicates, data are representative of more than three independent experiments). Background absorbance without protein was subtracted. B The determination of bacterial activities of serum, LSSL, and LSSL-depleted serum on E. coli and S. aureus after 12 or 24 h. Data are presented as the mean percentage ± SD of three independent experiments. C Scanning electron microscopy (SEM) analysis of E. coli and S. aureus after treatment with LSSL. E. coli and S. aureus were incubated with PBS and used as controls. The concentration of EDTA used was 50 μg/mL. Scale bar, 5 µm. D Agglutination of GFP-E. coli by LSSL. Different components were incubated with FITC-labeled E. coli (105 cells per well) in PBS for 1 h at room temperature and were examined using a high-content screening system (n = 3, ****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05)