Table 1 Data collection and refinement statistics for LSSL

EM equipment

Titan Krios (Thermo Fisher)

Voltage (kV)

300

Detector

Gatan K2 Summit

Energy filter

Gatan GIF Quantum, 20 eV

Pixel size (Å)

1.30654

Electron dose (e⁻Å⁻²)

50

Defocus range (µm)

~−1.5 to −2.5

Reconstruction software

RELION 3.1

Number of used particles

225,296

Symmetry

C1

Final masked resolution (Å)

3.344742

Map sharpening B-factor (Å2)

−137.37059

Model-building software

PDB code

coot

ID 7E1E