Fig. 4

UCK2 promoted cell cycle progression via regulating the expression of mTOR. A Volcano plot exhibited the gene expression altered after UCK2 knockdown. B The genes upregulated after UCK2 knockdown were enriched in the signalling pathway in the left panel; the genes downregulated are shown in the right panel. C Heatmap showed the expression of genes involved in ribosome biogenesis in the indicated cell lines. D Western blot (WB) examined the protein expression of p-mTOR and mTOR in the indicated cell lines. E The indicated cell lysates were prepared and immunoprecipitated with anti-UCK2 antibody; the protein expression of mTOR was examined by WB. F IHC detected the protein expression of UCK2 and mTOR in the left panel, and Pearson’s correlation analysis was used to determine the linear relationship between UCK2 and mTOR in the right panel. G CCK8 assays was used to determine the proliferation of the indicated cell lines; cells were treated with 10 μM rapamycin to block the mTOR signalling pathway. H Representative images for cell cycle assays in indicated cell lines in the left panel, and statistical analysis in the right panel. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001