Fig. 5

UCK2 accelerated the cell cycle progression via maintain the stable of PDPK1 mRNA. A The distribution of peaks on genes. B The intersection of genes targeted by UCK2 in 3′UTR and genes downregulated after UCK2 knocking down by shRNA. C The signalling pathways that genes of the intersection are enriched in. D The AU enriched regions on the UCK2 targeting site. E WB examined the protein expression of PDPK1, pAKT and AKT in the indicated cell lines. F The common signalling pathways that UCK2 and PDPK1 are related to. G CCK8 assays were used to determine the proliferation of the indicated cell lines. H Representative images for cell cycle assays in indicated cell lines in the left panel, and statistical analysis in the right panel. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001