Fig. 2

Inhibition of circFUT8 suppressed HCC cell malignancy and tumor growth. A CircFUT8 expression was reduced by sh-circFUT8#1/2 transfection in HCC cells. B, C Colony formation and EdU assays were conducted to verify the proliferation of HCC cells with the transfection of sh-circFUT8. D The invasion of HCC cells after circFUT8 depletion was evaluated by transwell assays. E The apoptosis rate of HCC cells after circFUT8 depletion was analyzed through flow cytometry. F–I In vivo tumor assays were performed to measure the tumor size, growth, volume, and weight after circFUT8 knockdown. The experimental group treated with sh-circFUT8#1/2 exhibited a significant statistical difference in comparison with the control sh-NC group, with a P-value < 0.01