Fig. 3

CircFUT8 sponged miR-552-3p to elevate CHMP4B expression. A Potential miRNAs of circFUT8 were forecast through starBase and the enrichment of selected miRNA candidates was measured in the bio-circFUT8 group via the RNA pull down assay. B MiR-552-3p expression in HCC cell lines relative to normal THLE-3 cells. C The favorable overexpression efficiency of miR-552-3p in HCC cells. D The binding sites between circFUT8 and miR-552-3p as well as the mutated sequences were predicted through starBase. E The luciferase activity of HCC cells transfected with miR-552-3p mimics and NC mimics in wild type and mutant circFUT8 vectors. F, G The influence of circFUT8 silencing or miR-552-3p overexpression on CHMP4B expression was assessed by luciferase reporter assay. H The binding sites and the mutated sequences between miR-552-3p and CHMP4B were forecasted through starBase. I The interaction among miR-552-3p, circFUT8, and CHMP4B in HCC was confirmed via luciferase reporter data. J CHMP4B expression in different groups was tested by RT–qPCR. In comparison with the negative control group, data in the experimental group was observed to be statistically significant (P < 0.01)