Skip to main content
Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: Exosomal miR-628-5p from M1 polarized macrophages hinders m6A modification of circFUT8 to suppress hepatocellular carcinoma progression

Fig. 5

M1 macrophages-derived exosomes reduced the m6A level of circFUT8 by inhibiting METTL14. A The possible m6A modification sites in the circFUT8 sequence. B The effect of M1-Exo on the m6A-modified circFUT8 in HCC cells was analyzed through the MeRIP assay. C RIP data of the enrichment of circFUT8 binding to YTHDC1 (the nuclear m6A reader) after adding M1-Exo in HCC cells. D The expression of five proteins that can modify RNA m6A (METTL3, METTL14, WTAP, FTO, and ALKBH5) was tested. E METTL14 expression was enhanced by pcDNA3.1/METTL14 transfection. F The effect of METTL14 overexpression on m6A-circFUT8 enrichment upon M1-Exo treatment was tested through the Me-RIP assay. G The interaction between YTHDC1 and circFUT8 upon M1-Exo treatment was determined through the RIP assay. In relevant assays, data in the experimental group was observed to be statistically significant compared with the negative control group (P < 0.01)

Back to article page

Cellular & Molecular Biology Letters

ISSN: 1689-1392

Contact us