Fig. 6

M1-Exo transferred miR-628-5p to inhibit METTL14 expression in HCC. A Potential miRNAs in the upstream of METTL14 were selected through bioinformatic predictions. B The expression of potential miRNAs following M1-Exo treatment was tested. C, D MiR-628-5p expression was enhanced or silenced in HCC cells, and then the expression of METTL14 was analyzed by RT–qPCR. E METTL14 expression was measured under different conditions. F The binding sites between METTL14 and miR-628-5p were forecast through starBase. G An RNA pull down assay was conducted to evaluate the enrichment of METTL14 in wild type or mutated bio-miR-628-5p groups. H The luciferase activity of HCC cells transfected with miR-628-5p mimics in the wild type or mutated METTL14 groups. In relevant assays, data in the experimental group was statistically significant in comparison with the negative control group (P value < 0.01)