Fig. 1From: PSMC3 promotes RNAi by maintaining AGO2 stability through USP14Four proteins interacting with AGO2 were screened and two promote RNAi. a Schematic of yeast two-hybrid system used to identify the AGO2 interaction proteins. b The interactive clones isolated from a human lung cDNA library were reconfirmed by cotransfection into naive cdc25H yeast with pSOS AGO2-PIWI or the following negative control plasmids: pSOS vector (containing the human SOS gene only), pSOS-MAFB (fusion of the hSOS gene and the transcription factor MAFB) and pSOS-Col I (fusion of the hSOS gene and type IV collagenase). No interaction was detected when the interactive clones were cotransfected with the control plasmids. Accession number of each positive gene is shown Back to article page