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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: Metabolic disorders sensitise endometrial carcinoma through endoplasmic reticulum stress

Fig. 4

Endoplasmic reticulum stress and Ca2+ homoeostasis are essential for promoting apoptosis by combined effect of insulin and palmitic acid. A–C Immunoblot of ER stress marker GRP78, p-eIF2α and CHOP after treating with 100 nM insulin for 0–24 h in Ishikawa, RL-952 and AN3CA cells. D–F Histogram plot shows change of ER stress marker with insulin treatment in EC cells relative to 0 h. *P < 0.05. G, H Immunoblot of ER stress marker GRP78, p-eIF2α and CHOP after treating with insulin, PA and insulin plus PA. Histogram plot shows change of ER stress marker with insulin and/or PA treatment *P < 0.05. I Immunoblot of GRP78, p-eIF2α and CHOP after PA plus insulin treatment with or without 4-phenylbutyric acid (4-PBA) pre-treatment. J Ca2+ store in endoplasmic reticulum after treatment with vehicle, 100 nM insulin alone, 0.5 mM PA alone or 0.5 mM PA plus 100 nM insulin for 6 h. K Apoptosis of Ishikawa cells detecting by Annexin V-FITC/PI labelling flow cytometry after 0.5 mM PA plus 100 nM insulin treatment for 12 h, with or without pretreatment with 4-phenylbutyric acid (4-PBA) or RU-360

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