Fig. 2

Inhibition of FPR2 reduces neutrophil infiltration in IRI kidneys. The left renal pedicle of the rat was clamped for 45 min to induce IRI. The right kidney was removed before releasing the clamps. WRW₄ was administered intravenously 30 min before surgery. Kidney tissues and peripheral blood were collected 24 h after surgery. A Immunochemistry staining of CD68⁺ macrophages and MPO⁺ neutrophils. Scale bar, 100 μm. Representative images from one experiment out of three are shown. B The percentages of CD45⁺CD11b⁺MPO⁺NE⁺ neutrophils in kidney tissues were determined by flow cytometry. C The percentages of CD45⁺CD11b⁺MPO⁺NE⁺ neutrophils in peripheral blood were determined by flow cytometry. D Levels of CD62L on neutrophils in peripheral blood. The results are representative of three independent experiments. **P < 0.01, ***P < 0.001. ns, P > 0.05