Fig. 3
mtFPs are dominative FPR2 agonists in the acute phase of IRI. The left renal pedicle of the rat was clamped for 45 min to induce IRI. The right kidney was removed before releasing the clamps. WRW4 was administered intravenously 30 min before surgery. Liver tissues, kidney tissues, and peripheral blood were collected at 0, 6, 12, and 24 h after surgery. A Illustration of FPR2 agonists in a rat model. B At 0, 6, 12, and 24 h after surgery, the levels of ANXA1, SAA, and β-actin in IRI rat liver and kidney tissues were determined by western blot analysis. C At 0, 6, 12, and 24 h after surgery, the levels of ANXA1 and SAA in IRI rat serum were measured via enzyme-linked immunosorbent assay. D At 0, 6, 12, and 24 h after surgery, the levels of LXA4 and RvD1 in IRI rat kidney and serum were measured via enzyme-linked immunosorbent assay. E At 0, 6, 12, and 24 h after surgery, rat kidney and serum samples were collected. Kidneys were dissected and finely minced in Hank’s buffer containing 10% type IV collagenase, and the supernatant was collected as kidney interstitial tissues. Levels of ND6 in IRI rat kidney interstitial tissues and serum were measured via enzyme-linked immunosorbent assay. F At 0 and 24 h after surgery, rat kidneys were collected for immunochemistry staining of ND6 expression in kidneys and to obtain electron microscopy images of mitochondria in tubular cells. *P < 0.05, **P < 0.01, ***P < 0.001. ns, P > 0.05.