Fig. 5
FPR2 inhibition alleviates systematic and local inflammation in IRI. The left renal pedicle of rats was clamped for 45 min to induce IRI. The right kidney was removed before releasing the clamps. WRW4 was administered intravenously 30 min before surgery. Kidney tissues and peripheral blood were collected 24 h after surgery. A MDA levels in rat kidneys were measured by thiobarbituric acid chromogenic reaction. B SOD levels in rat kidneys were measured by the WST-8 method. C Immunochemistry staining of MMP-9 expression in rat kidneys. Scale bar, 100 μm. Representative images from one experiment out of three are shown. D–F At 24 h after surgery, the levels of IL-1β, IL-1Ra, and IL-8 in rat kidneys and serum were measured using enzyme-linked immunosorbent assays. G At 24 h after surgery, the mRNA levels of IL-6, TNFα, IFN-γ IL-1β, IL-1Ra, and IL-8 in rat kidneys were measured via quantitative real-time PCR. Data are normalized to the expression levels in sham operation kidneys. *P < 0.05, **P < 0.01, ***P < 0.001. ns, P > 0.05