Fig. 7

PBM can inhibit the expression of TUG1, TLR3, and inflammatory cytokines in SCI mice. A, B RT-PCR was used to detect the expression of TUG1 and TLR3 at 1, 3, 7, 14, and 28 days after SCI. In addition, RT-PCR results showed that the expression of TUG1 and TLR3 decreased after 7 days of PBM treatment. C, D FISH assay was used to detect the expression of TUG1 in SCI group and PBM group at 1, 3, 7, 14, and 28 days after SCI. Scale bar, 200 μm. E The protein expression level of TLR3 was detected by western blotting at 1, 3, 7, 14, and 28 days after SCI. F PBM treatment reduces TLR3 expression. (G–K) RT-PCR was used to detect the expression of TNF-α, IL-1α, IL-1β, IL-6, and CXCL2 at 1, 3, 7, 14, and 28 days after SCI, and the expression of TNF-α, IL-1α, IL-1β, IL-6, and CXCL was reduced after 7 days of PBM treatment. n = 3 per group. *P < 0.05, ***P < 0.001, ***P < 0.001, ****P < 0.0001. ns not significant