Fig. 2

LRP6 inactivation inhibited osteogenic commitment in force-induced PDLSCs. a The protein expression of ALP, RUNX2, and PCNA in PDLSCs under CSS. b The mRNA expression of ALPL, RUNX2, and PCNA in PDLSCs under CSS. c The protein expression of P-LRP6 and LRP6 in the control, sh-NC, and sh-LRP6 group after stretching for 24 h. d The mRNA expression of LRP6 in the control, sh-NC, and sh-LRP6 group after stretching for 24 h. e The ratio of the protein levels of P-LRP6/total LRP6 in the control, sh-NC, and sh-LRP6 group after stretching for 24 h. f The protein expression of ALP, RUNX2, OSX, and PCNA in the control, sh-NC, and sh-LRP6 group after stretching for 24 h. g The mRNA expression of ALPL, RUNX2, OSX, and PCNA in the control, sh-NC, and sh-LRP6 group after stretching for 24 h. h, i Immunofluorescence and semi-quantitative analysis of Ki67⁺ cells in the control, sh-NC, and sh-LRP6 group after stretching for 24 h. Scale bar, 50 μm. j, k ALP staining and ALP activity analysis of the control, sh-NC, and sh-LRP6 group after stretching for 24 h. Scale bar, 200 μm. *P < 0.05, **P < 0.01, ***P < 0.001. N.S., no significance. Results are presented as mean ± SD of triplicated experiments