Skip to main content
Fig. 3 | Cellular & Molecular Biology Letters

Fig. 3

From: LRP6/filamentous-actin signaling facilitates osteogenic commitment in mechanically induced periodontal ligament stem cells

Fig. 3

LRP6 inactivation disrupted F-actin dynamics in force-induced PDLSCs. a Imaging cell shape after stretching for 24 h by optical microscopy. Scale bars, 100 μm and 50 μm. b Quantification of cell area, c roundness, and d width-to-length ratio for the control, sh-NC, and sh-LRP6 group after stretching for 24 h. e The mRNA levels of ROCK1, ACTB, and RHOA in the control, sh-NC, and sh-LRP6 group after stretching for 24 h. f The protein levels of β-Actin, F-actin, ROCK1, and RHOA in the control, sh-NC, and sh-LRP6 group after stretching for 24 h. g, h Phalloidin fluorescence staining and semi-quantitative analysis in the control, sh-NC, and sh-LRP6 group after stretching for 24 h. Scale bar, 20 μm. *P < 0.05, **P < 0.01, ***P < 0.001. Results are presented as mean ± SD of triplicated experiments

Back to article page