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Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: LRP6/filamentous-actin signaling facilitates osteogenic commitment in mechanically induced periodontal ligament stem cells

Fig. 5

LRP6/F-actin axis modulated YAP nuclear translocation and activation in force-induced PDLSCs. a, b Immunofluorescence and semi-quantitative analysis of YAP nuclear localization in the control, sh-NC, and sh-LRP6 group after stretching for 24 h. Scale bar, 50 μm. c, d Western blotting analysis indicated the ratio of P-YAP/total-YAP in the control, sh-NC, and sh-LRP6 group after stretching for 24 h. e The mRNA levels of YAP target genes (CTGF, TEAD1, TEAD2, TEAD3, TEAD4) in the control, sh-NC, and sh-LRP6 group after stretching for 24 h. f, g Immunofluorescence and semi-quantitative analysis of YAP nuclear localization in the DMSO and Cyto D + DMSO groups after stretching for 24 h. Scale bar, 50 μm. h, i Western blotting analysis indicated the ratio of P-YAP/total-YAP in the DMSO and Cyto D + DMSO groups after stretching for 24 h. j The mRNA levels of YAP target genes (CTGF, TEAD1, TEAD2, TEAD3, TEAD4) in the DMSO and Cyto D + DMSO groups after stretching for 24 h. *P < 0.05, **P < 0.01, ***P < 0.001. Results are presented as mean ± SD of triplicated experiments

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