Fig. 4
From: Outside the limit: questioning the distance restrictions for cooperative miRNA binding sites
Analysis of miRNA cooperativity by proteome data. Whole cell protein extracts from 293T human embryonic kidney cells were analyzed by high-resolution mass spectrometry upon (i) control plasmid transfection (con), (ii) overexpression of only miR-21-5p (miR21), (iii) only miR-155-5p (miR155) or (iv) upon co-expression of both miRNAs (miR21/155). Three independent transfection experiments were performed. As defined by the comparison of effect sizes by Cohen’s d, 22 gene products indicated cooperative miRNA effects (A; clustering of z-scored expression data). For specific representation, relative protein expression data for DDX17 (B), CYPN2 (C) and UBE3A (D) were normalized to controls and are shown as averaged results with SEM