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Fig. 1 | Cellular & Molecular Biology Letters

Fig. 1

From: Low-intensity pulsed ultrasound (LIPUS) enhances the anti-inflammatory effects of bone marrow mesenchymal stem cells (BMSCs)-derived extracellular vesicles

Fig. 1

Characterization of BMSCs and BMSCs-derived EVs. A BMSCs before and after LIPUS irradiation both displayed a representative spindle-like morphology. Scale bar: 50 μm. B Flow cytometry analysis of the cell surface markers on BMSCs. Cells were cultured in P5 or P6 and the results showed that BMSCs were positive for CD29, CD44 and Sca-1, but negative for CD11b, CD45 and CD34. C Morphology of C-EVs and LIPUS-EVs observed by TEM. Scale bar, 1 μm; inset scale bar, 200 nm. D Average size of BMSCs-derived EVs measured by NanoSight NTA. n = 5 per group. E Zeta potential of C-EVs and LIPUS-EVs detected using ZetaView. n = 3 per group. F The surface markers of BMSCs-derived EVs (CD9, CD63, CD81, TSG101 and Calnexin) were detected by western blotting. G Overall concentration of EVs by Nanosight NTA profiling revealing a significant increase in EVs following LIPUS exposure. **p < 0.01 vs. Control. n = 5 per group. Statistical significance assessed by unpaired two-tailed t-test. EVs, extracellular vesicles

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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