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Fig. 3 | Cellular & Molecular Biology Letters

Fig. 3

From: Low-intensity pulsed ultrasound (LIPUS) enhances the anti-inflammatory effects of bone marrow mesenchymal stem cells (BMSCs)-derived extracellular vesicles

Fig. 3

LIPUS strengthened the anti-inflammatory effect of BMSCs-derived EVs. A The protein expression levels of pro-inflammatory cytokines (IL-10 and NF-κB) in both C-EVs and LIPUS-EVs detected by ELISA assay (n = 6 per group). B The protein expression of IL-10 and NF-κB in RAW264.7 cells measured by ELISA. And the mRNA level of IL-10 and NF-κB in RAW264.7 cells by qRT-PCR. (n = 6 per group). C The protein expression of IL-10 and NF-κB in skin allografts measured by ELISA. The mRNA expression of IL-10 and IL-6 in skin allografts measured by qRT-PCR. (n = 4 per group). D H&E-stained sections of skin allograft. Scale bar = 50 μm. Immunohistochemistry staining of the skin allograft in control group contained massive infiltrates of CD3+, CD11b+ and CD68+ cells in comparison to the C-EVs and LIPUS-EVs group. (Representative images from 3 different mice per group). Scale bar = 100 μm. Error bars represent mean ± SD. ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. Statistical significance assessed by unpaired two-tailed t-test. EVs, extracellular vesicles

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