Fig. 2
From: Single-cell transcriptome analysis reveals liver injury induced by glyphosate in mice
Transcriptomic changes of HSC activation after GLY stimulation. A Volcano plot of genes differentially expressed between control and GLY-treated HSCs. B GO analysis for upregulated DEGs of total HSCs after GLY treatment. C UMAP plot showing the cell subtypes of HSCs. D The expression of specific marker genes identified HSC subtypes. E Merged UMAP plot for control and GLY groups. F Violin plots of the expression for three genes associated with ECM. G Pseudotime trajectory analysis of HSC subtypes. H Proportion changes of each cell type for different states during the pseudotime analysis. I Typical images and bar graph showing the increased area percentage of Desmin after GLY treatment. Scale bar, 100 μm; Error bar, mean ± standard deviation (SD); P value is from Student’s t test (unpaired and two-tailed); ****P ≤ 0.0001; the quantification was based on six random areas from each group