Fig. 5
From: Single-cell transcriptome analysis reveals liver injury induced by glyphosate in mice
Inflammatory response and pro-fibrosis of LCMs after GLY treatment. A UMAP visualization of the three subtypes for LCMs. B The expression of specific marker genes identified the subtypes of LCM. C The distribution of each cell type in control and GLY groups. D Pie plots showing proportional changes of each cell type for two groups. E GO analysis for upregulated DEGs of different cell subtypes. F Venn diagram displaying the numbers of upregulated DEGs in the three subtypes (left). GO analysis for unique upregulated DEGs of LCM2 after GLY treatment (right). G Violin plots showing the expression levels of inflammatory response-related genes in each subtype. H Violin plots showing the expression levels of ROS metabolic process-related genes in each subtype. I Violin plots showing the expression levels of Spp1 and Tgfbi genes in each subtype. P value is from wilcox.test (unpaired and two-tailed); **P < 0.001; NS, not significant. J Typical images and bar graph showing the increased area percentage of CD68 in the liver capsule after GLY treatment. The liver capsule outlined by yellow-dotted lines was denoted by Cx3cr1 staining; scale bar, 100 μm; error bar, mean ± SD; P value is from Student’s t-test (unpaired and two-tailed); ****P ≤ 0.0001; the quantification was based on eight random areas from each group