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Ribosomal DNA, tri- and bi-partite pericentromeres in the permanent translocation heterozygote Rhoeo spathacea

Cellular & Molecular Biology Letters volume 15, pages 651–664 (2010)Cite this article

Abstract

High- and low-stringency FISH and base-specific fluorescence were performed on the permanent translocation heterozygote Rhoeo spathacea (2n = 12). Our results indicate that 45S rDNA arrays, rDNA-related sequences and other GC-rich DNA fraction(s) are located within the pericentromeric regions of all twelve chromosomes, usually colocalizing with the chromomycin A3-positive bands. Homogenization of the pericentromeric regions appears to result from the concerted spread of GC-rich sequences, with differential amplification likely. We found new 5S rDNA patterns, which suggest a variability in the breakpoints and in the consequent chromosome reorganizations. It was found that the large 5S rDNA locus residing on each of the 8E and 9E arms consisted of two smaller loci. On each of the two chromosome arms 3b and 4b, in addition to the major subtelomeric 5S rDNA locus, a new minor locus was found interstitially about 40% along the arm length. The arrangement of cytotogenetic landmarks and chromosome arm measurements are discussed with regard to genome repatterning in Rhoeo.

Abbreviations

CMA3 + :

chromomycin A3 positive

FISH:

fluorescence in situ hybridization

NORs:

nucleolus organizer regions

PTH:

permanent translocation heterozygosity

rDNA:

ribosomal DNA

WATs:

whole arm translocations

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Author information

Authors and Affiliations

  1. Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian University, Grodzka 52, 31-044, Kraków, Poland

    Hieronim Golczyk

  2. Department of Plant Anatomy and Cytology, University of Silesia, Jagiellońska 28, 40-032, Katowice, Poland

    Robert Hasterok

  3. Department of Genetics, Plant Breeding and Seed Science, Agricultural University of Krakow, Al. 29 Listopada 54, 31-425, Kraków, Poland

    Marek Szklarczyk

Authors
  1. Hieronim Golczyk
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  2. Robert Hasterok
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  3. Marek Szklarczyk
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Correspondence to Hieronim Golczyk.

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Golczyk, H., Hasterok, R. & Szklarczyk, M. Ribosomal DNA, tri- and bi-partite pericentromeres in the permanent translocation heterozygote Rhoeo spathacea . Cell Mol Biol Lett 15, 651–664 (2010). https://doi.org/10.2478/s11658-010-0034-0

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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