Fig. 4

NH₄Cl addition and removal stimulate [Ca²⁺]_i changes in astrocytes. a, b and c – Changes after addition of 1 mM, 5 mM and 20 mM NH₄Cl plotted as trends. d, e and f – Changes after removal of 1 mM, 5 mM and 20 mM NH₄Cl plotted as trends; boxplots on each side present median, upper and lower quartile, minimum and maximum and outliers. T1 – time point before the substitution of the SBS with the NH₄Cl bathing solution; T2 – time point at which the maximum change of F₃₄₀/F₃₈₀ was reached after the substitution of the SBS with the NH₄Cl bathing solution; T3 – time point (at 900 s) before substituting the NH₄Cl bathing solution with the SBS; T4 – time point of the maximum change of F₃₄₀/F₃₈₀ after substituting the NH₄Cl bathing solution with the SBS. Experiments are numbered using consecutive numbers as performed