Changes in expression of cartilaginous genes during chondrogenesis of Wharton’s jelly mesenchymal stem cells on three-dimensional biodegradable poly(L-lactide-co-glycolide) scaffolds

Table 1 Sequences of primers used for real-time PCR

Gene	Primer sequences	Accession no.	Amplicon length (bp)
GAPDH	5’ GAAGGTGAAGGTCGGAGTC 3’	NM_002046.3	226
GAPDH	5’ GAAGATGGTGATGGGATTTC 3’	NM_002046.3	226
Collagen type I	5’ CCACCAATCACCTGCGTACA 3’	NM_000088	119
Collagen type I	5’ CATCGCACAACACCTTGCC 3’	NM_000088	119
Collagen type II	5’ TGCTGACGCTGCTCGTCGC 3’	NM_001844.4	163
Collagen type II	5’ TCGTCGCAGAGGACAGTCCCA 3’	NM_001844.4	163
Collagen type III	5’ CAGCAGGGTGCAATCGGCAGT 3’	NM_000090	177
Collagen type III	5’ TGGTTGCCCTGGGTGGCCT 3’	NM_000090	177
Aggrecan	5’ CAAGAGCAGTGCAATCGTTGG 3’	NM_001135.2	127
	5’ CAAGAGCAGTGCAATCGTTGG 3’	NM_013227.2
	5’ ACATTCAGCTGCGGTTCCG 3’	NM_013227.2

ISSN: 1689-1392