Fig. 3

Exo70 location in the process of normal A7r5 cell migration. a A7r5 cells stably expressing GFP-tagged Exo70 were stained for α-actin (green), Exo70 (red) and nucleus (blue). The image on the right shows α-actin, Exo70, and the nucleus overlap. The yellow area indicates the overlap of α-actin and Exo70. The arrow points to α-actin and Exo70 together in the migration wound edges. Scale length is 50 μm. b Exo70 expression in A7r5 rat Exoc7-K1 and A7r5 rat Exoc7-D2 cells examined by western blot. β-actin was used as a loading control. c Quantification of Exo70 expression. ***p < 0.001. d Exo70 expression in the above siRNA-silenced cells was examined by qPCR. Relative Exo70 expression was normalized to untreated A7r5-control cells. ***p < 0.001