Table 2 HTF isolation using 5% FGF-EMEM
Description of each isolation step | Passage number | Culture dish used | Time needed to reach monolayer | Features of obtained monolayer |
|---|---|---|---|---|
Biopsy cutting and setting up of the isolation | 0 | Well of 12-well plate | 15.3 ± 2.1 days | Mostly HTFs, possible single epithelial cells |
Cell detachment using trypsin and cell scraper | 1 | One 25 cm2 T-flask | 7.3 ± 0.6 days | Mostly HTFs |
Cell detachment using trypsin | 2 | Two 25 cm2 T-flasks | 2 days | 99–100% of vimentin-positive HTFs |
Trypsinization and cryopreservation of HTFs | - | - | - | - |