Table 4 Key times during 3 parallel isolations using 5% FGF-EMEM and 10% DMEM with medium change to 5% FGF-EMEM
Type of sample | Time from biopsy to passage 1 (days) | Time from passage 1 to passage 2 (days) | Time of complete procedure, from biopsy to cryopreservation (days) |
|---|---|---|---|
Sample 1/5% FGF-EMEM | 17 | 7 | 26 |
Sample 2/5% FGF-EMEM | 13 | 8 | 23 |
Sample 3/5% FGF-EMEM | 16 | 7 | 25 |
Mean ± SD | 15.3 ± 2.1 | 7.3 ± 0.6 | 24.7 ± 1.5 |
Sample 1/10% DMEM/5% FGF-EMEM | 14 | 30 | 46 |
Sample 2/10% DMEM/5% FGF-EMEM | 10 | failure | failure |
Sample 3/10% DMEM/5% FGF-EMEM | 16 | 27 | 45 |
Mean ± SD | 13.3 ± 3.1 | 28.5 ± 2.1 | 45.5 ± 0.7 |