Fig. 1
From: ABCC6 knockdown in HepG2 cells induces a senescent-like cell phenotype
Effect of ABCC6 knockdown on GSH/GSSG ratio and ROS generation. a – The GSH/GSSG ratio was determined using the glutathione fluorometric assay, as described in the Materials and Methods section. The results are reported as means ± SD of three independent experiments performed in triplicate. b – ROS production was detected via FACS using a DCFH-DA fluorescent probe as described in the Materials and Methods section. Scr-shRNA: HepG2 cells stably transfected with scrambled shRNA; ABCC6-shRNA: HepG2 cells stably transfected with ABCC6-shRNA. The values are the means ± SD of three replicates from three independent experiments. **p < 0.01. Unpaired data were assessed for statistical significance using Student’s t test