Fig. 3
Knockdown of H19 suppressed the differentiation of bovine tibia skeletal muscle (sk-muscle) satellite and C2C12 cells. a The immunofluorescence results for C2C12 cells and satellite cells on day 4 of differentiation. b The silencing efficiency of pLenti-H19. Cells with pLenti-NTC were the negative control and wild-type cells were the blank control (control). c The differentiation rate of C2C12 cells and the satellite cells on days 1, 2, 3, 4 and 5. (d, e, f and g). Detection of myoblast marker and myoblast inhibitory genes based on the protein level after H19 was silenced by pLenti-H19 vector transfection in bovine sk-muscle satellite cells (d, e) and C2C12 cells (f, g). h, i RT-PCR results for bovine sk-muscle satellite cells (h) and C2C12 cells (i) after H19 was silenced by pLenti-H19 vector transfection. j, k. mRNA level of bovine sk-muscle satellite cells (j) and C2C12 cells (k) after H19 was silenced by pLenti-H19 vector transfection. pLenti-NTC was the negative control. The blank control was the mRNA of satellite and C2C12 cells without any treatment, with cultivation for the same number of days. GAPDH was internal control. *p < 0.05, **p < 0.01