Fig. 2

Influences of miR-378 on the proliferation of colon cancer cells. a The MTT assay was performed to measure the amount of SW480 and HCT-116 cells transfected with miR-378 mimics or ASO-miR-378. b A colony formation assay was performed to detect SW480 and HCT-116 cells transfected with miR-378 mimics or ASO-miR-378. c FACS was performed to determine the level of cell apoptosis when miR-378 was overexpressed or knocked down. d Western blot was used to detect the apoptosis proteins Bad and Bcl-2 in SW480 cells transfected with miR-378 mimics. GAPDH was used as a loading/transfer control and for normalization of the values. *p < 0.05, **p < 0.01, ***p < 0.001