Fig. 4

The effects of ADAM23 on the neurite length, as measured via stereological analysis. a–f Immunohistochemistry images of cells transfected with pCA-GFP (a, b), pCA-A23 (c, d), and pGS-A23 (e, f), showing the neurite length of Tuj-1-positive cells 3 days (d3; a, c, e) and 7 days (d7; b, d, f) after the initiation of differentiation. Arrows indicate the Tuj-1-positive neurites (green). Cell nuclei are colored blue. Scale bar: 50 μm for all. g, h Quantification of the neurite length measured using a stereological analytical method after transfection with pCA-A23 (g) or pGS-A23 (h). The measurements were taken on d3 and d7 after the initiation of differentiation. Data are presented as mean ± SEM from at least three independent experiments. *p < 0.05 and ***p < 0.001