Fig. 4

The effect of LPS on ERK 1/2, p38 and JNK phosphorylation in time- and dose-dependent response. H9c2 cells were placed in 6-well plates. Cells were stimulated with LPS (1 μg/ml) for the times and doses indicated in the figure. Cell lysates were fractionated via SDS-PAGE and analyzed via western Blotting using an antibody against phospho-ERK 1/2 (a), −p38 (b) or -JNK (c). Membranes were then stripped and reprobed using an antibody against each kinase as a control for equal loading. The data are representative of one of three independent experiments performed with similar results. Phosphorylation was quantified via densitometry with LabWorks 4.0 (Upland, CA, USA) commercial software. The results are expressed as means ± S.D. *p < 0.05 vs. untreated group