Fig. 3

Hypoxia induces miR-200b in human airway epithelial cells in a HIF-1-dependent manner. a The predicted target site of miR-200b in CFTR 3′UTR is shown above. The miR-200b target site was predicted in human CFTR 3′UTR only, Hypoxia-induced changes in the expression profiles of miR-200b and miR-200c in Calu3 and 16HBE14o- cells are shown. The miRNA levels were monitored in qRT-PCR experiments. The results from 2 independent experiments (n = 8) are plotted normalized to RNU48 and expressed as a fold-change over the normoxic control. b Calu3 and 16HBE14o- cells were treated with hypoxia mimetic (200 μM CoCl₂ for 12 h) and the miRNA levels were monitored in qRT-PCR experiments. miR-200b and miR-200c levels were measured in 3 independent experiments (n = 10) and are plotted relative to RNU44 levels and expressed as a fold change over the untreated controls. Error bars represent standard deviations (SD). Significant changes (P < 0.05) are marked with an asterisk