Fig. 4
From: Collagen-derived dipeptide prolyl-hydroxyproline promotes osteogenic differentiation through Foxg1
![Fig. 4](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs11658-017-0060-2/MediaObjects/11658_2017_60_Fig4_HTML.gif)
Effect of Pro-Hyp on proliferation and ALP activity in Foxg1-knockdown cells. a – Foxg1 was knocked down in MC3T3-E1 cells, which were then cultured with or without 0.1 mM Pro-Hyp for 2 days. MC3T3-E1 cell proliferation was measured using the WST-1 assay. b – Foxg1 was knocked down in MC3T3-E1 cells, which were then cultured with or without 0.1 mM Pro-Hyp for 5 days. The scanned images show ALP staining in MC3T3-E1 cells. c – The ALP staining area was evaluated using Image J software. Data are presented as means ± SD (n = 4). Bars not sharing a letter differ, p < 0.05