Fig. 2
From: GSK-3β-mediated regulation of cadmium-induced cell death and survival
![Fig. 2](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs11658-018-0076-2/MediaObjects/11658_2018_76_Fig2_HTML.gif)
Cadmium increases intracellular Ca2+ concentration in neuronal cells. SH-SY5Y cells were treated with or without 10 μM BAPTA-AM for 30 min and then incubated with 25 μM Cd for the indicated times. a – Ca2+ concentration was quantified using the calcium indicator dye, Fluo-4 AM. b – GRP78 was analyzed via western blotting after 30 min pretreatment with 10 μM BAPTA-AM, followed by 12 h incubation with 25 μM Cd. c – Apoptotic cells were counted using the annexin V assay. The data are expressed as the means ± SEM of the apoptotic cells from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001