Fig. 3

Akt and GSK-3β regulation by cadmium. SH-SY5Y cells were treated with 25 μM Cd for the indicated times. Total cellular proteins were extracted and separated using SDS-PAGE. a – Western blot analysis was performed using anti-phospho-Akt (T308), S473 and phospho-GSK-3β (S9) antibodies. Band intensities were quantified based on densitometric values using Fujifilm Science Lab 97 Image Gauge software (version 2.54). The data are from at least three independent experiments. b, c – p-AKT and p-GSK-3β were analyzed via western blotting after 30 min pretreatment with 10 μM BAPTA-AM (b) or 1 μM wortmannin (c), followed by 12 h incubation with 25 μM Cd. d – Apoptotic cells were counted using the annexin V assay. The data are expressed as the means ± SEM of the percentage of apoptotic cells from at least three independent experiments. *p < 0.05, **p < 0.01