Fig. 1

Morphology and characterization of MSCs cultured in medium supplemented with 5% PL. a Photos of cells at 150× magnification. i) Migrating cells appeared at the edge of the explants after 7 days. ii) Fibroblast-like morphology of MSCs in passage 3. b Flow cytometry of UCMSCs cultured in medium supplemented with 5% PL. FSC and SSC distribution of gated cells (i). The cells stained positive for CD90 (ii), CD105 (iii) and CD44 (iv), but negative for CD34 (v) and CD45 (vi). c Adipogenic and osteogenic differentiation of MSCs. Differentiated cells stained with alizarin red (i and ii) or sudan III (iii and iv). i and iii show the controls; ii shows positive for mineralization indicating osteogenic differentiation; iv shows positive staining for lipid vacuoles indicating adipogenic differentiation. The flow cytometry data are representative from one out of three donors tested (n = 3)