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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: Mitochonic acid 5 activates the MAPK–ERK–yap signaling pathways to protect mouse microglial BV-2 cells against TNFα-induced apoptosis via increased Bnip3-related mitophagy

Fig. 2

MA-5 preserved the mitochondrial function. a and b The mitochondrial potential was measured via JC1 staining in control cells (Ctrl), and in cells treated with 10 ng/ml TNFα (TNFα), or 10 ng/ml TNFα and 5 μM MA-5 (TNFα+MA-5). c and d The mitochondrial calcium level was assessed as fluorescence intensity in control cells (Ctrl), and in cells treated with 10 ng/ml TNFα (TNFα), or 10 ng/ml TNFα and 5 μM MA-5 (TNFα+MA-5). e The rate of mPTP opening was determined in control cells (Ctrl), and in cells treated with 10 ng/ml TNFα (TNFα), or 10 ng/ml TNFα and 5 μM MA-5 (TNFα+MA-5). f and g The immunofluorescence assay for HtrA2/Omi in cells treated with 10 ng/ml TNFα (TNFα), or 10 ng/ml TNFα and 5 μM MA-5 (TNFα+MA-5). h through n Western blots were used to analyze the impact of MA-5 and TNFα treatment on apoptotic proteins in cells treated with 10 ng/ml TNFα (TNFα), or 10 ng/ml TNFα and 5 μM MA-5 (TNFα+MA-5). *p < 0.05 vs. control (Ctrl) group; #p < 0.05 vs. TNFα group

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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