The norpurpureine alkaloid from Annona purpurea inhibits human platelet activation in vitro

Table 1 Norpurpureine prevents ADP-induced platelet adhesion onto fixed fibrinogen

	Net Adhesion (AU)		Platelet-specific adhesion (PSA) to Fg induced by ADP	PSA (%) vs. vehicle
	ADP (−)	ADP (+)	Platelet-specific adhesion (PSA) to Fg induced by ADP	PSA (%) vs. vehicle
Vehicle	0.39 (0.04)	0.95 (0.05)	0.56 (0.05)	100.00 (4.7)
Norp 220 μM	0.42 (0.03)	0.51 (0.04)	0.09 (0.01)	16.07 (1.8)^***
Norp 90 μM	0.41 (0.02)	0.73 (0.04)	0.32 (0.02)	56.55 (3.7)^***
Norp 30 μM	0.42 (0.03)	0.85 (0.02)	0.43 (0.03)	76.78 (4.7)^**
Apyrase	0.40 (0.02)	0.49 (0.03)	0.09 (0.02)	16.67 (2.7)^***

Platelets in PRP (1 × 10⁵) were pretreated with vehicle (DMSO 0.25%), apyrase (0.5 U/ml) or norpurpureine (Norp) at the indicated concentrations for 10 min before the addition of 10 μM ADP to test their adhesion onto fixed fibrinogen (Fg). The values are the means ± SD (n = 3, done in triplicate) p < 0.01 (**) and p < 0.001 (***) compare to vehicle.

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