Fig. 3

Osthole alleviates IL-1β-induced proliferation and migration in SW982 human synovial cell line. a, b To evaluate the effect of osthole on SW982 survival and on IL-1β-treated SW982 cells, SW982 cells were incubated with the indicated concentration of osthole (0–200 μM) alone for 24 and 48 h (A) or together with 10 ng/ml IL-1β in 96-well plates for 48 h. MTT assay was applied to measure SW982 survival. c Morphology of normal SW982 cells, 10 ng/ml IL-1β treated SW982 cells and 10 ng/ml IL-1β treated SW982 cells incubated with 50, 100 μM osthole for 48 h. d SW982 cells were incubated with IL-1β and osthole for 48 h and migration of SW982 cells was detected using Transwell assay. Representative images of crystal violet-stained migratory SW982 on the membrane. *p < 0.05, **p < 0.01 compared with the normal group, ^#p < 0.05, ^##p < 0.01 compared with the 10 ng/ml IL-1β group