Fig. 6

The effects of miR-495 on high glucose-induced NF-κB and TGF-β1/Smad signaling pathways. CFs were transfected with miR-495 mimic for 48 h, and then treated with 5.5 or 25 mM glucose for 24 h. a The protein expressions of p-IKK, t-IKK, p-IκBα, t-IκBα, p-NF-κB, t-NF-κB, p-Smad3, t-Smad3 and PAI-1 were determined via western blot. b The secreted TGF-β1 proteins in the supernatants of CFs were detected using ELISA assays. All data are presented as means ± SEM (n = 4). **p < 0.01 vs. control; ^##p < 0.01 vs. vehicle + high glucose. The vehicle is transfection reagent alone