Fig. 1

The expressions of ATF3 and miR-488 in TSCC tissues and cell lines. a Quantitative RT-PCR analysis of ATF3 expression in TSCC tissues (n = 30) and adjacent normal tissues (n = 10). Transcript levels were normalized to GAPDH expression. b Relative ATF3 expression analyzed via quantitative RT-PCR in five TSCC cell lines normalized to GAPDH (n = 6). c Quantitative RT-PCR analysis of miR-488 level in TSCC tissues and adjacent normal tissues. Transcript levels were normalized to U6 level. d Relative miR-488 level analyzed via quantitative RT-PCR in five TSCC cell lines normalized to U6 (n = 6). e Pearson’s correlation analysis of the relative expression levels of miR-488 and the relative ATF3 mRNA levels in TSCC tissues. All data are presented as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 vs. normal tissues or NHOK