Fig. 2

The effects of ATF3 silencing on invasion and EMT in TSCC cells. TCA8113 cells were transfected with si-ATF3 or si-NC. a The mRNA and protein expressions of ATF3 were determined via quantitative RT-PCR and western blot, respectively. b Invasion was assessed via transwell assay. c The protein expressions of MMP-2, MMP-7 MMP-9, TIMP-1 and TIMP-2 were assessed via western blot and ELISA assays. d The expressions of E-cadherin, N-cadherin and vimentin were determined via quantitative RT-PCR and western blot assays, respectively. All data are presented as means ± SEM, n = 6. ^#p < 0.05, ^##p < 0.01 vs. si-NC